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KMID : 0613820080180030344
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Journal of Life Science
2008 Volume.18 No. 3 p.344 ~ p.349
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Comparison of Cellular Senescence Phenotype in Human Fibroblasts from New-born and Aged Donors.
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Lee Hye-Won
Hwang Eun-Seong
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Abstract
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Normal somatic cells proliferate for a limited number of doublings in culture and then enter an irreversible growth-arrest state called replicative senescence. Replicative senescence has been believed a reason for the limited cellular turnover and deterioration of tissue function in aged animals. However, there is no experimental evidence supporting this assumption. Furthermore, cells from aged person have been poorly characterized with an exception of the cases of T cells. In this study, we examined cell biological changes occurring in replicative senescence of fibroblast strains originated from a new-born (NHF-NB) and a 87 year old man (NHF-87). NHF-87 (and the cells from a 75-year old) proliferated to smaller population doublings and with longer doubling times than NHF-NB did. At early passages, NHF-87 exhibited a low senescence-associated β-Gal (SA β-Gal ) activity and lipofuscin level, typical markers for cellular senescence. Furthermore, they maintained low levels of lysosome and reactive oxygen species (ROS). All of these levels increased dramatically in the late passage NHF-87 quite similarly as those in the late passaged NHF-NB did. These results indicate that most cells originated from the aged maintain a phenotype of the cells originated from new-born donors and undergo replicative senescence with the same kinetics as that of the cells from new-born. It is also indicated that not SA β-Gal activity but cell proliferation rate may be qualified as a biomarker for cells aged in vivo.
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KEYWORD
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Senescence-associated , β-galactosidase, activity (SA β-Gal , activity), replicative senescence, aging, reactive oxygen species, lysosome
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